human duo set elisa kits  (R&D Systems)

Journal: Redox Biology

Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC

doi: 10.1016/j.redox.2026.104172

Figure Lengend Snippet: Characterization of IL-1α overexpression constructs in Cal27 HNSCC cells. Gene expression (A-C), protein release (D) and protein expression (E-G) of the three IL1A constructs – Full- Length (FL), N-terminal (NT) and C-terminal (CT) in the Cal27 IL-1α-overexpressing cells were analyzed by RT-qPCR, ELISA and Western blot respectively compared to control. GAPDH was used as an endogenous control for PCR analysis (A-C); α-Tubulin was used as the loading control for whole cell lysate and cytoplasmic fraction western blots (E-F); and Lamin B1 was used as the loading control for the nuclear fraction Western blot (G). Bars represent mean ± SEM from n = 3 independent experiments. IL-1α band intensities were normalized to the respective loading controls. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

Article Snippet: Cell culture media from IL-1α overexpressed Cal27 cells were collected for analyzing the levels of IL-1α, IL-1β, IL-6, IL-8 or IL-1RA using Human Duo Set ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer's protocols.

Techniques: Over Expression, Construct, Gene Expression, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: Redox Biology

Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC

doi: 10.1016/j.redox.2026.104172

Figure Lengend Snippet: IL-1α overexpression alters expression of IL-1 pathway genes. Gene expression of IL1B (A), IL6 (B), IL8 (C), IL1RN (D), IL1RAP (E) and IL1R1 (F) in the three IL1A constructs – Full-Length (FL), N-terminal (NT) and C-terminal (CT) in Cal27 IL-1αoverexpressing cells were analyzed by RT-PCR using GAPDH as an endogenous control. Protein secretion of IL-1β (G), IL-6 (H), IL-8 (I), and IL1RA (J) in cell culture supernatants was quantified by ELISA with protein concentrations normalized to cell numbers. Whole cell lysates were analyzed for IL-1R1 expression by Western blot using GAPDH as a loading control (K). Bars represent mean ± SEM from n = 3 independent experiments.∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.

Article Snippet: Cell culture media from IL-1α overexpressed Cal27 cells were collected for analyzing the levels of IL-1α, IL-1β, IL-6, IL-8 or IL-1RA using Human Duo Set ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer's protocols.

Techniques: Over Expression, Expressing, Gene Expression, Construct, Reverse Transcription Polymerase Chain Reaction, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: The Journal of Obstetrics and Gynaecology Research

Article Title: Impact of tumor necrosis factor‐alpha and lysophosphatidic acid on the behavior of ovarian cancer cells in a three‐dimensional collagen hydrogel

doi: 10.1111/jog.70026

Figure Lengend Snippet: VEGF secretion in response to TNF‐α or LPA at two different concentrations. The concentration of VEGF was measured with ELISA in SKOV‐3 (a and b), OVCAR‐8 (c and d), OVCAR‐5 (e and f), and OVCAR‐4 (g and h) cell lines grown in collagen gels for 2–8 days with the appropriate concentration of vehicle (control gels), TNF‐α or LPA. Protein concentrations of VEGF accumulated over 48 h in the culture medium are shown as mean + SEM from ≥3 independent experiments. Statistical significance p * <0.05; ** <0.01; **** <0.0001. LPA, lysophosphatidic acid; TNF‐α, tumor necrosis factor‐alpha; VEGF, vascular endothelial growth factor.

Article Snippet: Protein levels were measured using the human VEGF, human IL‐8, or human IL‐6 using the appropriate Duo SET ELISA development kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer's protocols.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: The Journal of Obstetrics and Gynaecology Research

Article Title: Impact of tumor necrosis factor‐alpha and lysophosphatidic acid on the behavior of ovarian cancer cells in a three‐dimensional collagen hydrogel

doi: 10.1111/jog.70026

Figure Lengend Snippet: IL‐8 secretion in response to TNF‐α or LPA at two different concentrations. The concentration of IL‐8 was measured with ELISA in SKOV‐3 (a and b), OVCAR‐8 (c and d), OVCAR‐5 (e and f), and OVCAR‐4 (g and h) cell lines grown in collagen gels for 2–8 days with the appropriate concentration of vehicle (control gels), TNF‐α or LPA. Protein concentrations of IL‐8 accumulated over 48 h in the culture medium are shown as mean + SEM from ≥3 independent experiments. Statistical significance p * <0.05; ** <0.01; *** <0.001; **** <0.0001. IL‐8, interleukin‐8; LPA, lysophosphatidic acid; TNF‐α, tumor necrosis factor‐alpha.

Article Snippet: Protein levels were measured using the human VEGF, human IL‐8, or human IL‐6 using the appropriate Duo SET ELISA development kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer's protocols.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: The Journal of Obstetrics and Gynaecology Research

Article Title: Impact of tumor necrosis factor‐alpha and lysophosphatidic acid on the behavior of ovarian cancer cells in a three‐dimensional collagen hydrogel

doi: 10.1111/jog.70026

Figure Lengend Snippet: IL‐6 secretion from cells that were stimulated by two concentrations of TNF‐α or LPA. The concentration of IL‐6 was measured with ELISA in SKOV‐3 (a and b), OVCAR‐8 (c and d), OVCAR‐5 (e and f), and OVCAR‐4 (g and h) cell lines grown in collagen gels for 2–8 days with the appropriate concentration of vehicle (control gels), TNF‐α or LPA. Protein concentrations of IL‐6 accumulated over 48 h in the culture medium are shown as mean + SEM from ≥3 independent experiments. Note the different scales in the left versus right columns. Statistical significance * <0.05 ** <0.01 *** <0.001 **** <0.0001. Results for OVCAR‐5 with LPA (f) were below detection. IL‐6, interleukin‐6; LPA, lysophosphatidic acid; TNF‐α, tumor necrosis factor‐alpha.

Article Snippet: Protein levels were measured using the human VEGF, human IL‐8, or human IL‐6 using the appropriate Duo SET ELISA development kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer's protocols.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control